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separate grna expression plasmids  (OriGene)


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    Structured Review

    OriGene separate grna expression plasmids
    Separate Grna Expression Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pcas-guide/pCas-Guide/bio_rxiv__64898__2026__05__07__723586-113-10-15
    Average 95 stars, based on 75 article reviews
    separate grna expression plasmids - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Knockout of key receptors (PDGFRA and NRP2) in the guinea pig model blocks direct and endocytic pathways of CMV cell entry.
    Article Snippet: Exon- specific gRNAs were designed via the online programme (http://www.rgenome.net/) to avoid off- target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas- Guide, OriGene) for each target gene. ..

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model
    Article Snippet: Exon 2-specific guide RNA (gRNA) was designed via an online program ( www.rgenome.net ) to avoid off-target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide-EF1a-GFP; OriGene): pR1 (5′-GGTGTGGGCCGCCGAGGCGT-3′), pR2 (5′-TCTGGGAGAGTTCCCCGACG-3′), and pR3 (5′-CGTTTCTGATGTCCACGTCG-3′). .. GEFh cells in 6-well plates were transduced with defective lentivirus expressing Cas9 under HCMVIE enhancer control (Origene) to enable expression of Cas9 and subsequently transfected with the gRNA expression plasmids (pR1, pR2, and pR3).

    Article Title: Selective knockout of key CMV receptors in fetal cells blocks direct and endocytic pathways of entry in the guinea pig
    Article Snippet: Exon 2-specific guide RNA (gRNA) was designed via an online program ( www.rgenome.net ) to avoid off-target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide-EF1a-GFP; OriGene): pR1 (5′-GGTGTGGGCCGCCGAGGCGT-3′), pR2 (5′-TCTGGGAGAGTTCCCCGACG-3′), and pR3 (5′-CGTTTCTGATGTCCACGTCG-3′). .. GPL cells in 6-well plates were transduced with defective lentivirus expressing Cas9 under HCMVIE enhancer control (Origene) to enable expression of Cas9 and subsequently transfected with the gRNA expression plasmids (pR1, pR2, and pR3).

    Article Title: Selective knockout of key CMV receptors in fetal cells blocks direct and endocytic pathways of entry in the guinea pig
    Article Snippet: .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide, OriGene) for each target gene. ..

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model
    Article Snippet: .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide, OriGene) for each target gene. ..

    Control:

    Article Title: Knockout of key receptors (PDGFRA and NRP2) in the guinea pig model blocks direct and endocytic pathways of CMV cell entry.
    Article Snippet: Exon- specific gRNAs were designed via the online programme (http://www.rgenome.net/) to avoid off- target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas- Guide, OriGene) for each target gene. ..

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model
    Article Snippet: Exon 2-specific guide RNA (gRNA) was designed via an online program ( www.rgenome.net ) to avoid off-target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide-EF1a-GFP; OriGene): pR1 (5′-GGTGTGGGCCGCCGAGGCGT-3′), pR2 (5′-TCTGGGAGAGTTCCCCGACG-3′), and pR3 (5′-CGTTTCTGATGTCCACGTCG-3′). .. GEFh cells in 6-well plates were transduced with defective lentivirus expressing Cas9 under HCMVIE enhancer control (Origene) to enable expression of Cas9 and subsequently transfected with the gRNA expression plasmids (pR1, pR2, and pR3).

    Article Title: Selective knockout of key CMV receptors in fetal cells blocks direct and endocytic pathways of entry in the guinea pig
    Article Snippet: Exon 2-specific guide RNA (gRNA) was designed via an online program ( www.rgenome.net ) to avoid off-target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide-EF1a-GFP; OriGene): pR1 (5′-GGTGTGGGCCGCCGAGGCGT-3′), pR2 (5′-TCTGGGAGAGTTCCCCGACG-3′), and pR3 (5′-CGTTTCTGATGTCCACGTCG-3′). .. GPL cells in 6-well plates were transduced with defective lentivirus expressing Cas9 under HCMVIE enhancer control (Origene) to enable expression of Cas9 and subsequently transfected with the gRNA expression plasmids (pR1, pR2, and pR3).

    Article Title: Selective knockout of key CMV receptors in fetal cells blocks direct and endocytic pathways of entry in the guinea pig
    Article Snippet: .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide, OriGene) for each target gene. ..

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model
    Article Snippet: .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide, OriGene) for each target gene. ..

    Expressing:

    Article Title: Knockout of key receptors (PDGFRA and NRP2) in the guinea pig model blocks direct and endocytic pathways of CMV cell entry.
    Article Snippet: Exon- specific gRNAs were designed via the online programme (http://www.rgenome.net/) to avoid off- target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas- Guide, OriGene) for each target gene. ..

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model
    Article Snippet: Exon 2-specific guide RNA (gRNA) was designed via an online program ( www.rgenome.net ) to avoid off-target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide-EF1a-GFP; OriGene): pR1 (5′-GGTGTGGGCCGCCGAGGCGT-3′), pR2 (5′-TCTGGGAGAGTTCCCCGACG-3′), and pR3 (5′-CGTTTCTGATGTCCACGTCG-3′). .. GEFh cells in 6-well plates were transduced with defective lentivirus expressing Cas9 under HCMVIE enhancer control (Origene) to enable expression of Cas9 and subsequently transfected with the gRNA expression plasmids (pR1, pR2, and pR3).

    Article Title: Selective knockout of key CMV receptors in fetal cells blocks direct and endocytic pathways of entry in the guinea pig
    Article Snippet: Exon 2-specific guide RNA (gRNA) was designed via an online program ( www.rgenome.net ) to avoid off-target sites. .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide-EF1a-GFP; OriGene): pR1 (5′-GGTGTGGGCCGCCGAGGCGT-3′), pR2 (5′-TCTGGGAGAGTTCCCCGACG-3′), and pR3 (5′-CGTTTCTGATGTCCACGTCG-3′). .. GPL cells in 6-well plates were transduced with defective lentivirus expressing Cas9 under HCMVIE enhancer control (Origene) to enable expression of Cas9 and subsequently transfected with the gRNA expression plasmids (pR1, pR2, and pR3).

    Article Title: Selective knockout of key CMV receptors in fetal cells blocks direct and endocytic pathways of entry in the guinea pig
    Article Snippet: .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide, OriGene) for each target gene. ..

    Article Title: Cytomegalovirus pentamer dependent endocytic cellular infection utilizes redundant entry receptors in the guinea pig model
    Article Snippet: .. DNA sequences were cloned under US6 promoter control in three separate gRNA expression plasmids (pCas-Guide, OriGene) for each target gene. ..

    Plasmid Preparation:

    Article Title: KDM6B safeguards mineralized tissue homeostasis from mechanical stress through epigenetic control of PIEZO1-mediated mechanotransduction in the mouse incisor
    Article Snippet: These gRNAs were similarly cloned into the pCas-Guide-Puro-CRISPRi vector to generate a custom Piezo1 CRISPRi kit (Origene Technologies, CW312380 ). .. The pCas-Guide-Puro-CRISPRi-Scramble vector (Origene Technologies, GE100084 ) was used as a negative control. .. Primary cells isolated from control mouse incisors were transfected with the indicated CRISPRi constructs using TurboFectin 8.0 Transfection Reagent (Origene Technologies, TF81001).

    Article Title: Bispecific DNA‐Peptide Probes for Targeting Receptor Pairs on Live Cells
    Article Snippet: For later restriction and ligation into a pAAVS1puro-DNR vector (GE100024, OriGene) for knock-in transfection, the customized sequence was equipped with overhangs compatible with KpnI and NotI restriction sites to enable transfer by restriction ligation into the pAAVS1-puro-DNR vector (Fig. S8), which provides the pAAVS1-eGFP-MS2BP-NLS-P2A-4NE.GLRP-MS2 vector (Fig. S9). .. The stable cell line was generated using HEK293 cells by co-transfection (Fig. S10) of the previously designed transgene knock-in vector and pCas-Guide-AAVS1 (GE100023, OriGene) for targeted insertion into AAVS1 locus (Adeno-associated virus integration site 1, human Chromosome 19). .. For transfection of 1 g of plasmid DNA, Lipofectamine LTX plus from Invitrogen (Carlsbad, USA) was used.

    Negative Control:

    Article Title: KDM6B safeguards mineralized tissue homeostasis from mechanical stress through epigenetic control of PIEZO1-mediated mechanotransduction in the mouse incisor
    Article Snippet: These gRNAs were similarly cloned into the pCas-Guide-Puro-CRISPRi vector to generate a custom Piezo1 CRISPRi kit (Origene Technologies, CW312380 ). .. The pCas-Guide-Puro-CRISPRi-Scramble vector (Origene Technologies, GE100084 ) was used as a negative control. .. Primary cells isolated from control mouse incisors were transfected with the indicated CRISPRi constructs using TurboFectin 8.0 Transfection Reagent (Origene Technologies, TF81001).

    CRISPR:

    Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
    Article Snippet: .. An all-in-one CRISPR/Cas9 vector with tdTomato was created by replacing the GFP fragment of the pCas-Guide-EF1a-GFP vector (Origene, Rockville, MD, USA) with a tdTomato fragment amplified via PCR, using the BmgBI and ClaI sites. ..

    Amplification:

    Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
    Article Snippet: .. An all-in-one CRISPR/Cas9 vector with tdTomato was created by replacing the GFP fragment of the pCas-Guide-EF1a-GFP vector (Origene, Rockville, MD, USA) with a tdTomato fragment amplified via PCR, using the BmgBI and ClaI sites. ..

    Polymerase Chain Reaction:

    Article Title: Distinct TAM subset with cross-dressing capability determines the bifurcation of tumor immunity.
    Article Snippet: .. An all-in-one CRISPR/Cas9 vector with tdTomato was created by replacing the GFP fragment of the pCas-Guide-EF1a-GFP vector (Origene, Rockville, MD, USA) with a tdTomato fragment amplified via PCR, using the BmgBI and ClaI sites. ..

    Stable Transfection:

    Article Title: Bispecific DNA‐Peptide Probes for Targeting Receptor Pairs on Live Cells
    Article Snippet: For later restriction and ligation into a pAAVS1puro-DNR vector (GE100024, OriGene) for knock-in transfection, the customized sequence was equipped with overhangs compatible with KpnI and NotI restriction sites to enable transfer by restriction ligation into the pAAVS1-puro-DNR vector (Fig. S8), which provides the pAAVS1-eGFP-MS2BP-NLS-P2A-4NE.GLRP-MS2 vector (Fig. S9). .. The stable cell line was generated using HEK293 cells by co-transfection (Fig. S10) of the previously designed transgene knock-in vector and pCas-Guide-AAVS1 (GE100023, OriGene) for targeted insertion into AAVS1 locus (Adeno-associated virus integration site 1, human Chromosome 19). .. For transfection of 1 g of plasmid DNA, Lipofectamine LTX plus from Invitrogen (Carlsbad, USA) was used.

    Generated:

    Article Title: Bispecific DNA‐Peptide Probes for Targeting Receptor Pairs on Live Cells
    Article Snippet: For later restriction and ligation into a pAAVS1puro-DNR vector (GE100024, OriGene) for knock-in transfection, the customized sequence was equipped with overhangs compatible with KpnI and NotI restriction sites to enable transfer by restriction ligation into the pAAVS1-puro-DNR vector (Fig. S8), which provides the pAAVS1-eGFP-MS2BP-NLS-P2A-4NE.GLRP-MS2 vector (Fig. S9). .. The stable cell line was generated using HEK293 cells by co-transfection (Fig. S10) of the previously designed transgene knock-in vector and pCas-Guide-AAVS1 (GE100023, OriGene) for targeted insertion into AAVS1 locus (Adeno-associated virus integration site 1, human Chromosome 19). .. For transfection of 1 g of plasmid DNA, Lipofectamine LTX plus from Invitrogen (Carlsbad, USA) was used.

    Knock-In:

    Article Title: Bispecific DNA‐Peptide Probes for Targeting Receptor Pairs on Live Cells
    Article Snippet: For later restriction and ligation into a pAAVS1puro-DNR vector (GE100024, OriGene) for knock-in transfection, the customized sequence was equipped with overhangs compatible with KpnI and NotI restriction sites to enable transfer by restriction ligation into the pAAVS1-puro-DNR vector (Fig. S8), which provides the pAAVS1-eGFP-MS2BP-NLS-P2A-4NE.GLRP-MS2 vector (Fig. S9). .. The stable cell line was generated using HEK293 cells by co-transfection (Fig. S10) of the previously designed transgene knock-in vector and pCas-Guide-AAVS1 (GE100023, OriGene) for targeted insertion into AAVS1 locus (Adeno-associated virus integration site 1, human Chromosome 19). .. For transfection of 1 g of plasmid DNA, Lipofectamine LTX plus from Invitrogen (Carlsbad, USA) was used.

    Virus:

    Article Title: Bispecific DNA‐Peptide Probes for Targeting Receptor Pairs on Live Cells
    Article Snippet: For later restriction and ligation into a pAAVS1puro-DNR vector (GE100024, OriGene) for knock-in transfection, the customized sequence was equipped with overhangs compatible with KpnI and NotI restriction sites to enable transfer by restriction ligation into the pAAVS1-puro-DNR vector (Fig. S8), which provides the pAAVS1-eGFP-MS2BP-NLS-P2A-4NE.GLRP-MS2 vector (Fig. S9). .. The stable cell line was generated using HEK293 cells by co-transfection (Fig. S10) of the previously designed transgene knock-in vector and pCas-Guide-AAVS1 (GE100023, OriGene) for targeted insertion into AAVS1 locus (Adeno-associated virus integration site 1, human Chromosome 19). .. For transfection of 1 g of plasmid DNA, Lipofectamine LTX plus from Invitrogen (Carlsbad, USA) was used.



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